MicroRNA <em>In situ</em> Hybridization for Formalin Fixed Kidney Tissues
نویسندگان
چکیده
منابع مشابه
MicroRNA in situ hybridization for formalin fixed kidney tissues.
In this article we describe a method for colorimetric detection of miRNA in the kidney through in situ hybridization with digoxigenin tagged microRNA probes. This protocol, originally developed by Kloosterman and colleagues for broad use with Exiqon miRNA probes(1), has been modified to overcome challenges inherent in miRNA analysis in kidney tissues. These include issues such as structure iden...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2013
ISSN: 1940-087X
DOI: 10.3791/50785